abstract：:Experimentation has been carried out to study proliferation of plasma cells in the chicken Harderian gland (HG) and to determine if a HG factor influences immune cell (i.e., B cell) proliferation. In young chickens, flow cytometric analysis of propidium iodide (PI)-stained plasma cells revealed that the percentages of...

journal_title：Microscopy research and technique

authors： Scott TR,Savage ML

更新日期：1996-06-01 00:00:00

abstract：:Fatty acid and alkyl compositions of 1-alkyl-2,3-diacylglycerols (ADG) in the Harderian gland of the golden hamster were determined. Fatty acids of male ADG consisted of straight chain saturated acids ranging from C12 to C22. Both even- and odd-numbered acids were observed, indicating that acetyl- and propionyl-CoAs w...

journal_title：Microscopy research and technique

authors： Seyama Y,Otsuka H,Ohashi K,Vivien-Roels B,Pevet P

更新日期：1996-05-01 00:00:00

abstract：:As part of ongoing studies aimed at clarifying the early events of bone matrix deposition and mineralization, we have characterized primary osteoblast cultures using ultrastructural and immunocytochemical methods. Osteogenic cells were isolated by sequential enzymatic digestion of newborn rat (2-4-day-old) calvariae a...

journal_title：Microscopy research and technique

authors： Nanci A,Zalzal S,Gotoh Y,McKee MD

更新日期：1996-02-01 00:00:00

abstract：:Scanning electron microscope images of complete and damaged frustules of Nitzschia marina Grun. revealed an unusual chambered structure of the valve. This structure is not present in any existing nitzschioid genus and warrants an independent generic status for this taxon, which is therefore separated into Alveus gen. ...

journal_title：Microscopy research and technique

authors： Kaczmarska I,Fryxell GA

更新日期：1996-01-01 00:00:00

abstract：:In this paper we present the state of knowledge on cell-cell interactions in the testis of two groups of anamniote vertebrates--teleosts and elasmobranchs--which include most fish. In these fish, the structural organization of the testis differs fundamentally from that which characterizes amniotes in which the germina...

journal_title：Microscopy research and technique

authors： Loir M,Sourdaine P,Mendis-Handagama SM,Jégou B

更新日期：1995-12-15 00:00:00

abstract：:Spermatogenesis is a process of division and differentiation by which spermatozoa are produced in seminiferous tubules. A measure of efficiency of spermatogenesis is the estimated number of spermatozoa produced per day per gram of testicular parenchyma. This measure is not influenced by species differences in testicul...

journal_title：Microscopy research and technique

authors： Johnson L

更新日期：1995-12-01 00:00:00

abstract：:Progressive multifocal leukoencephalopathy is an important viral opportunistic infection of oligodendrocytes leading to direct demyelination. Virus is likely disseminated to the brain via the blood. However, the timing of that dissemination with relationship to clinical disease is unknown. Important clues about viral ...

journal_title：Microscopy research and technique

authors： Aksamit AJ Jr

更新日期：1995-11-01 00:00:00

abstract：:Parathyroid glands (n = 271) removed from 130 patients were examined by light and electron microscopy. A standardized method of tissue processing was employed and morphometry was performed. The aim of the paper is to provide a description of the human parathyroid chief cell ultrastructure in health and disease, with q...

journal_title：Microscopy research and technique

authors： Cinti S,Sbarbati A

更新日期：1995-10-01 00:00:00

abstract：:Studies on embryonic development in vitro as well as observations in vivo, suggested that two aspects of oviduct physiology are important for early development. On one side has to be considered the oviduct "environment": temperature, pH, osmotic pressure, nutrients, oxygen tension, free radical scavengers, etc. On the...

journal_title：Microscopy research and technique

authors： Gandolfi F

更新日期：1995-09-01 00:00:00

abstract：:The cyclic fluctuations in circulating levels of 17 beta-estradiol and progesterone that occur during the menstrual or estrous cycle are responsible for dramatic, cyclic changes in the epithelial lining and secretory status of the mammalian oviduct. The timely transition in the synthesis and release of oviduct protein...

journal_title：Microscopy research and technique

authors： Murray MK,DeSouza MM,Messinger SM

更新日期：1995-08-15 00:00:00

abstract：:This paper describes the use of a photobleach method and confocal microscopy to compare the cell-to-cell transfer rate of 5,6 carboxyfluorescein in dissociated embryonic chick lens cells with those in the anterior epithelium of the whole embryonic chick lens. The average cell-to-cell transfer rates obtained were 7.9 x...

journal_title：Microscopy research and technique

authors： Miller A

更新日期：1995-08-01 00:00:00

abstract：:Ultramicrotomy has found novel application in materials research using transmission electron microscopy, for both analytical and high resolution work. Specimens 20-50 nm in thickness with lateral dimensions of up to 100 microns may routinely be prepared, with the additional advantage of precise area location for cross...

journal_title：Microscopy research and technique

authors： Glanvill SR

更新日期：1995-07-01 00:00:00

abstract：:This article focusses on the structural development of human esophageal ciliated epithelium. A combination of transmission electron microscopic (TEM), scanning electron microscopic (SEM), radioautographic, and light microscopic (LM) analyses were carried out using intact fetal tissues between 8 and 20 weeks of gestati...

journal_title：Microscopy research and technique

authors： Ménard D

更新日期：1995-06-15 00:00:00

abstract：:Uranium-contaminated soils from the U.S. Department of Energy (DOE) Fernald Site, Ohio, have been examined by a combination of backscattered electron imaging (BSE) and analytical electron microscopy with electron diffraction (AEM). The inhomogeneous distribution of particulate uranium phases in the soil required the d...

journal_title：Microscopy research and technique

authors： Buck EC,Dietz NL,Bates JK

更新日期：1995-06-01 00:00:00

abstract：:During the past few years, cyto- and immunocytochemical techniques have been developed and widely used for locating and identifying various molecules in plant cell compartments. The last decade has witnessed tremendous improvements in molecular cytology, thus allowing an accurate in situ detection of various component...

journal_title：Microscopy research and technique

authors： Benhamou N

更新日期：1995-05-01 00:00:00

abstract：:The preparation of optically clear, thick sections of fragile embryonic tissues greatly aids the power of confocal scanning laser microscopy in imaging three-dimensional structures. We report here conditions for embedding, sectioning, and staining embryos in polyacrylamide gels for a variety of confocal imaging techni...

journal_title：Microscopy research and technique

authors： Germroth PG,Gourdie RG,Thompson RP

更新日期：1995-04-15 00:00:00

abstract：:A new method to study the developing heart was developed. Using this "section directed" cryosectioning method, appropriate fixed embryos can be trimmed optimally to obtain sectional planes that, if necessary, can be matched with histologically treated sections. As a result, the morphological information obtained with ...

journal_title：Microscopy research and technique

authors： Seo JW,Kim EK,Brown NA,Wessels A

更新日期：1995-04-15 00:00:00

abstract：:The increasing importance of in vitro models has presented new challenges in SEM processing techniques. The present study has evaluated the quality of preservation of cultured human lens epithelial cells processed by critical point, Peldri II, and tert-butyl alcohol drying. Specimens processed by critical point drying...

journal_title：Microscopy research and technique

authors： Cantu-Crouch D,Howe WE,McCartney MD

更新日期：1995-04-01 00:00:00

abstract：:The epididymal junctional complex between adjacent principal cells is composed of apically located gap, adherens and tight junctions. Tight junctions between adjacent epithelial cells lead to the formation of the blood-epididymal barrier. The objectives of this study were to examine the structure of the epididymal jun...

journal_title：Microscopy research and technique

authors： Cyr DG,Robaire B,Hermo L

更新日期：1995-01-01 00:00:00

abstract：:Changes in the intracellular Ca2+ concentration ([Ca2+]i) within CA1 hippocampal pyramidal neurons were imaged using confocal laser scanning microscopy in conjunction with Ca(2+)-sensitive fluorescent indicators. The imaging was performed in thick hippocampal brain slices while simultaneously measuring or controlling ...

journal_title：Microscopy research and technique

authors： Jaffe DB,Brown TH

更新日期：1994-11-01 00:00:00

abstract：:An analytical result is given for the wavelength at which two or more first order Laue zone (FOLZ) lines intersect at a single point on the positive x axis when a face centred cubic crystal is observed in the convergent beam mode with the beam axis parallel to the [100] direction. The position of the intersection alon...

journal_title：Microscopy research and technique

authors： Curzon AE

更新日期：1994-10-15 00:00:00

abstract：:Superior cervical ganglion neurons (SCGNs) were isolated from 7-day-old rat SCG and cultured in MEM containing horse serum, fetal calf serum, and nerve growth factor. In this culture condition, it is well known that the SCGNs form cholinergic synapse. In 3-4 weeks cultured neurons, immunofluorescent staining for synap...

journal_title：Microscopy research and technique

authors： Mochida S,Nonomura Y,Kobayashi H

更新日期：1994-10-01 00:00:00

abstract：:In spite of accumulating evidence for a modulation of sympathetic neurotransmission by endogenously produced nitric oxide (NO), it remains unclear in which parts of the vascular system and at what level this interaction takes place. The aim of the present study was to investigate the distribution of endothelial and ne...

journal_title：Microscopy research and technique

authors： Addicks K,Bloch W,Feelisch M

更新日期：1994-10-01 00:00:00

abstract：:Ultra-thin frozen sections are ideal substrates for immunolabelling in high resolution electron microscopy. However, visualization of subcellular structures is inferior to that obtained with corresponding plastic sections. Although negative staining is generally effective and even superior to positive staining, the ac...

journal_title：Microscopy research and technique

authors： Himmelhoch S

更新日期：1994-09-01 00:00:00

abstract：:High-angle annular dark-field or Z-contrast microscopy was used to demonstrate that well dispersed metal supported catalysts consist of nanometer sized clusters. Depending upon the impregnated metal, different cluster sizes were observed. Grouping of Pd clusters could also be confirmed by analytical electron microscop...

journal_title：Microscopy research and technique

authors： Bradley SA,Cohn MJ,Pennycook SJ

更新日期：1994-08-01 00:00:00

abstract：:The present study provides experimental data which indicate that the neutrophil is ideal for studying programmed cell death or apoptosis in vitro. Neutrophils can be obtained from human peripheral blood in large numbers with minimal experimental manipulation and are easily separated from other leukocytes, providing ne...

journal_title：Microscopy research and technique

authors： Payne CM,Glasser L,Tischler ME,Wyckoff D,Cromey D,Fiederlein R,Bohnert O

更新日期：1994-07-01 00:00:00

abstract：:Basement membranes (BMs) were first described in the mid-19th century, but they were not isolated and prepared for compositional studies until nearly 100 years later. Early methods of isolation were carried out on renal glomeruli, which were first sub-fractionated from kidney tissues by sieving. BMs were then isolated...

journal_title：Microscopy research and technique

authors： Carlson EC

更新日期：1994-06-15 00:00:00

abstract：:Effective protocols for controlling crystal structure, size, and morphology attract considerable interest given the requirement for particles of modal size and shape in many areas of materials fabrication and the importance of crystallochemical selectivity in determining the exploitable properties of inorganic solids....

journal_title：Microscopy research and technique

authors： Heywood BR

更新日期：1994-04-01 00:00:00

abstract：:Cytochrome c oxidase is a complex integral membrane protein consisting of 13 different polypeptide chains and four metal centers having a total molecular weight of approximately 200,000 daltons. It can be isolated in two 2-dimensional crystalline forms differing in aggregation state of the enzyme. One crystal form con...

journal_title：Microscopy research and technique

authors： Frey TG

更新日期：1994-03-01 00:00:00

abstract：:Follicular development in the mammalian ovary is a complex process regulated by an orchestrated action of the pituitary gonadotropins, e.g., follicle stimulating hormone (FSH) and luteinizing hormone (LH), and local ovarian factors, such as peptide growth factors and steroids. The mechanism of endocrine/paracrine/auto...

journal_title：Microscopy research and technique

authors： Roy SK

更新日期：1994-02-01 00:00:00

abstract：:We compared physiological and ultrastructural indices of acute lung injury (ALI) during septic shock caused by taxonomically diverse pathogens to distinguish ALI due to endogenous inflammatory mediators vs. microbial exotoxins or other factors. Conscious rats were infected i.v. with gram-negative Escherichia coli (EC,...

journal_title：Microscopy research and technique

authors： Lechner AJ,Ryerse JS,Matuschak GM

更新日期：1993-12-01 00:00:00

abstract：:Ascidian embryos are useful for examining how events that occur during fertilization and cleavage affect gastrulation because they gastrulate early in development, during the seventh cleavage. In ascidians, both dorsal-ventral and anterior-posterior axes are determined before first cleavage. The dorsal-ventral axis is...

journal_title：Microscopy research and technique

authors： Swalla BJ

更新日期：1993-11-01 00:00:00

abstract：:The fine structure of the taste buds of circumvallate papillae of two strains of mice was studied by electron microscopy. Mice anesthetized with ketamine were perfused through the heart with a double aldehyde mixture in cacodylate buffer and the tissues embedded in Epon. Semi-serial sections were employed. The morphol...

journal_title：Microscopy research and technique

authors： Murray RG

更新日期：1993-10-15 00:00:00

abstract：:We have used high voltage electron microscopy and computer-generated three-dimensional reconstructions from serial sections to elucidate the structure of taste bud cells and their associated synapses in fungiform taste buds of the mouse. Five fungiform taste buds (two of which were serially sectioned) were examined wi...

journal_title：Microscopy research and technique

authors： Kinnamon JC,Henzler DM,Royer SM

更新日期：1993-10-01 00:00:00

abstract：:This work provides examples of some of the imaging capabilities of environmental scanning electron microscopy applied to easily charged samples relevant to particle analysis. Environmental SEM (also referred to as high pressure or low vacuum SEM) can address uncoated samples that are known to be difficult to image. Mo...

journal_title：Microscopy research and technique

authors： Wight SA,Zeissler CJ

更新日期：1993-08-01 00:00:00

abstract：:The grain boundary healing behavior of crushed rock salt was mainly studied by employing the environmental scanning electron microscope (ESEM) to study the consolidation mechanism of rock salt backfill. Dedicated miniature round rock salt specimens were prepared for observation of the water trapping effect by using a ...

journal_title：Microscopy research and technique

authors： Hwang CL,Wang ML,Miao S

更新日期：1993-08-01 00:00:00

abstract：:This study was designed to investigate patterns of fibrils organization in histochemically stained otoconia. Transmission electron microscope and video imaging were used. These data indicate that otoconia of the chick (Gallus domesticus) inner ear may have central cores in vivo. The data also show that the ultrastruct...

journal_title：Microscopy research and technique

authors： Fermin CD

更新日期：1993-07-01 00:00:00

abstract：:The metrial gland develops in the uterus of many rodent species during normal pregnancy. It is a maternally-derived tissue that contains stromal and vascular elements plus a population of large cells, striking in their light microscopic appearance due to the presence of numerous cytoplasmic granules. These cells, whic...

journal_title：Microscopy research and technique

authors： Croy BA,Kiso Y

更新日期：1993-06-15 00:00:00

abstract：:MIS, as a differentiate and antiproliferative agent, is precisely regulated, for example, at the transcriptional level by such transacting factors as SRY, and posttranslationally by testosterone. Processing of MIS most likely requires an as yet unknown in vivo protease which probably serves to control cleavage of MIS ...

journal_title：Microscopy research and technique

authors： Catlin EA,MacLaughlin DT,Donahoe PK

更新日期：1993-06-01 00:00:00

abstract：:In situ hybridization (ISH) for HIV is an arduous, demanding means of detecting viral genetic material in cells and tissues. Good ISH requires broad technical skills and devotion to controls for every step of the process as well as a critical eye when interpreting results. ISH may be used to detect HIV in three ways: ...

journal_title：Microscopy research and technique

authors： Fox CH,Cottler-Fox M

更新日期：1993-05-01 00:00:00